FTIR Spectroscopic Study of Aloe vera barbadensis Mill Buds

The objective of this research is to investigate number and types of bioactive compounds present in Aloe Vera buds grown in North East Punjab region of Pakistan by FTIR which is simple, nondestructive, cost effective and user friendly. Aloe vera was cultivated under normal atmospheric conditions. After two years, buds (early stage of flowers) were grown on twigs. The buds were plucked and blended with distilled water in National Juicer machine to have a concentrated blend. The blend was filtered to get clear solution, mixed with ethyl acetate and then solvent extracted. The organic part was isolated and dried at water bath at 60°C. Dried sample was analyzed using FTIR spectroscopic analysis. All activities were performed consecutively to avoid photochemical changes. The characteristic FTIR spectral lines have shown different characteristic peaks that correspond to different functional groups indicating presence of bioactive compounds like substituted cyclic alkanes, alkenes, alcohols, phenols, and aromatics etc. have been investigated.