Mayengbam, Shyamchand and Aachary, Ayyappan and Thiyam-Holländer, Usha (2014) Endogenous Phenolics in Hulls and Cotyledons of Mustard and Canola: A Comparative Study on Its Sinapates and Antioxidant Capacity. Antioxidants, 3 (3). pp. 544-558. ISSN 2076-3921
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Abstract
Endogenous sinapic acid (SA), sinapine (SP), sinapoyl glucose (SG) and canolol (CAN) of canola and mustard seeds are the potent antioxidants in various lipid-containing systems. The study investigated these phenolic antioxidants using different fractions of canola and mustard seeds. Phenolic compounds were extracted from whole seeds and their fractions: hulls and cotyledons, using 70% methanol by the ultrasonication method and quantified using HPLC-DAD. The major phenolics from both hulls and cotyledons extracts were SP, with small amounts of SG, and SA with a significant difference of phenolic contents between the two seed fractions. Cotyledons showed relatively high content of SP, SA, SG and total phenolics in comparison to hulls (p < 0.001). The concentration of SP in different fractions ranged from 1.15 ± 0.07 to 12.20 ± 1.16 mg/g and followed a decreasing trend- canola cotyledons > mustard cotyledons > mustard seeds > canola seeds > mustard hulls > canola hulls. UPLC-tandem Mass Spectrometry confirmed the presence of sinapates and its fragmentation in these extracts. Further, a high degree of correlation (r = 0.93) was noted between DPPH scavenging activity and total phenolic content.
Item Type: | Article |
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Subjects: | Open Research Librarians > Agricultural and Food Science |
Depositing User: | Unnamed user with email support@open.researchlibrarians.com |
Date Deposited: | 29 Jun 2023 04:08 |
Last Modified: | 31 Oct 2023 06:19 |
URI: | http://stm.e4journal.com/id/eprint/1356 |