Maruyama, Kazuichi and Yoneda, Kazuhito and Sugita, Sunao and Yamamoto, Yoshimi and Koike, Masato and Peters, Christoph and Uchiyama, Yasuo and Nishida, Kohji (2021) CTLA-2 Alpha Is a Potent Inhibitor of Angiogenesis in Murine Ocular Tissue. Antioxidants, 10 (3). p. 456. ISSN 2076-3921
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Abstract
Cytotoxic T lymphocyte antigen-2 (CTLA-2) alpha has been reported to suppress the activities of cathepsin L (Cath L), which is deeply involved in angiogenesis. Therefore, we assessed whether CTLA-2 alpha plays a role in angiogenesis in ocular tissue. To establish models of corneal inflammation and experimental choroidal neovascularization (CNV), male C57BL/6J mice (n = 5) underwent corneal suture placement or laser-induced CNV, respectively. Mice were then injected with recombinant CTLA-2 alpha (1 µg) into the peritoneal cavity at day 0 and every 2 days after operation. In vitro experiments were performed to assess the inflammatory response by measuring TNF-alpha secretion in peritoneal cavity exudate cells (PECs) or the proliferation of mouse vascular endothelial cells (mVECs). CTLA-2 alpha treatment dramatically suppressed corneal angiogenesis, as well as laser-induced CNV. Moreover, CTLA-2 alpha inhibited the proliferation of mVECs in vitro, while CTLA-2 alpha abolishment was able to rescue proliferation. However, CTLA-2 alpha could not suppress cytokine secretion from inflammatory cells such as PECs. In summary, CTLA-2 alpha was able to suppress angiogenesis by suppressing endothelial cell proliferation. Further studies are needed to investigate its usefulness as a new antiangiogenic treatment for a variety of conditions, including age-related macular degeneration.
Item Type: | Article |
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Subjects: | Open Research Librarians > Agricultural and Food Science |
Depositing User: | Unnamed user with email support@open.researchlibrarians.com |
Date Deposited: | 06 Jul 2023 04:49 |
Last Modified: | 30 Oct 2023 05:16 |
URI: | http://stm.e4journal.com/id/eprint/1401 |