abdallah, omnia mohamed and EL-Baghdady, Khaled Z. and Khalil, Mostafa M.H. and El Borhamy, Mervat I. and Meligi, Gamal (2019) Biosynthesis, Characterization and Cytotoxicity of Selenium Nanoparticles Using Bacillus tropicus Ism 2. Egyptian Academic Journal of Biological Sciences, G. Microbiology, 11 (1). pp. 47-57. ISSN 2090-0880
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Abstract
Green biosynthesis of selenium nanoparticles (SeNPs) using Bacillus tropicus Ism 2 (MK332444) isolated from soil polluted with industrial wastewater near the Ismailia Canal, Egypt, was carried out. SeNPs were biogenically synthesized using bacterial pellets mixed with 1 mM Na2SeO3. Characterization of biosynthesized SeNPs was carried out by UV-Vis spectrum where SeNPs solution exhibited characteristic peak at 270 nm. Dynamic Light Scattering (DLS) analysis and High-Resolution Transmission Electron Microscope (HRTEM) showed spherical shaped SeNPs with size ranged from 60 to 125 nm with PDI= 0.544 and zeta potential value of -30.6. Biologically synthesized SeNPs showed no antibacterial activity against both Gram-positive and Gram-negative bacteria. Cytotoxicity of SeNPs was studied against Huh-7 human liver cell line and showed low cytotoxicity with IC50 value of367±5.5 μg/ml. In conclusion in this work Bacillus tropicus Ism 2 locally isolated bacterium showed the capability to biosynthesize SeNPs with spherical shape and low cytotoxicity.
Item Type: | Article |
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Subjects: | Open Research Librarians > Biological Science |
Depositing User: | Unnamed user with email support@open.researchlibrarians.com |
Date Deposited: | 15 Jul 2023 05:51 |
Last Modified: | 09 Oct 2023 06:34 |
URI: | http://stm.e4journal.com/id/eprint/1429 |