Ameliorate and Standardization of the In vitro Micropropagation of Night-blooming Jasmine (Cestrum nocturnum L.)

Night-Blooming Jasmine is one of the most important ornamental plants all over the world. But, inadequate multiplication rate and disease transmittance hamper it’s cultivation to adjoin the demand of high quality planting material for commercial production. Therefore, an efficient in vitro micropropagation protocol is considered as one of the best options to overcome this problem. The present study was carried out to evaluate the micropropagation of Cestrum nocturnum using single nodes and shoot tips extracted from soft cuttings using MS nutrient media, 30.5 gl-1 Sucrose, 7.5 gl-1 agar and various concentrations of phyto-hormones in culture medium. The findings revealed that the exploitation of mercuric chloride (0.05%, HgCl2) for 10 minutes was very effective in preventing contamination and gave the highest survival percentage (99%). Maximum response (100%) was achieved at initiation stage from lateral bud explants on MS nutrient medium supplemented with 1.75 mgl-1 of BA with most NAA concentration. Significant difference in multiplication stage was observed between lateral buds & terminal buds, since lateral buds assembled large number of newer shoots & leaves in addition to longer new shoots. At the rooting stage, the treatment with 1 mgl-1 IBA gave the highest percentage (%) of rooting (100%), the highest amount of roots (14.1 root/explants) and the lengthy roots (8.48 cm) in-particular, on half strength MS medium. Finally plantlets were transferred into pots and acclimatized with 95% success. The key role of the present study on plant tissue and cell culture of C. nocturnum in meeting the growing needs and requirements of human beings in the fields of agriculture, forestry, horticulture and medicine is very impressive.