The Bioactivities of Garcinia xanthochymus for Cosmetics

Tyrosinase is the primary enzyme involved in the manufacture of melanin. The quantity of melanin generated by melanocyte cells influences skin color. As a result, the skin of the body requires substances that can suppress the activity of the tyrosinase enzyme. Garcinia xanthochymus is an Indonesian plant that is high in quercetin. LDL cholesterol levels can be reduced by using ethanol bark extract. G. xanthochymus xanthones have anti-diabetic properties. The goal of this study was to assess the potential and anti-elastase activity of G. xanthochymus fruit extracts extracted in non-hexane and methanol. The dried samples were constantly macerated in n-hexane and methanol. Furthermore, the tyrosinase inhibitory activity of each extract was determined using a microplate reader 96 well spectrophotometers set to 490 nm and a substrate of 3,4-dihydroxy-L-phenylalanine, L-DOPA. The results showed that the results of the Inhibitory Activity test at a concentration of 100 ppm of each extract were 35.41 ± 11.10% (hexane extract), and 28.83 ± 6.77% (methanol extract). extract). At 100 ppm, the percentage inhibition of the positive control (kojic acid) was 65.07 0.03 %. The spectrophotometric elastase enzyme inhibition test was performed by reacting the extract with porcine pancreatic elastase enzyme and utilizing the substrate N-Succ-(Ala)-3-p-nitroanilide (SANA). A microplate reader was used to detect absorbance at a wavelength of 405 nm. The enzyme inhibitory activity of 100 ppm n-hexane extract of orange peel extract was 65.17 6.44 %, whereas pericarp methanol extract was 29.81 10.67 %. Meanwhile, as a positive control, the inhibitory activity of the enzyme quercetin elastase was measured. The findings indicated that hexane and methanol extracts of G. xanthochymus pericarp have promise as skin lightening agents, with hexane extract yields being higher than methanol extract yields and tyrosinase inhibitory activity half that of kojic acid. Furthermore, the extract of G. xanthochymus n-hexane pericarp shows extremely high activity as an inhibitor of the elastase enzyme and has the potential to be employed as a component in cosmetics.