BIOSYNTHESIS AND ANTIBACTERIAL EFFECT OF SILVER NANOPARTICLE LOADED ON Glycyrrihza glabra

This study was designed to evaluate the antibacterial activity of green synthetic nanoparticles of Glycyrrihza glabra aqueous extract loaded with silver nitrate. Green nanoparticles were synthesized by mixing the plant aqueous extract with different silver nitrate (AgNO3) concentrations (1 mM, 1.5 mM, 1.75 mM, and 2 Mm) then detected by visual observation and UV visible spectroscopy through monitoring the color changing from yellowish to brown within 10 min and to dark brown after 1 h, which gave indication for the creation of silver nanoparticles. A characteristic and definite SPR (surface Plasmon resonance) band for green nanoparticles was obtained at around 433 nm. The results indicated that SPR peak of green nanoparticles maximum peak intensity was obtained at 1.5 mM of AgNO3. Atomic Force Microscopy analysis was used to characterize the shape of green nanoparticles which declared that the green synthetic nanoparticles had different average size depend on sliver concentrations (12.84,39.32, 28.82, 9.90 nm) for (1 mM, 1.5 mM, 1.75 mM and 2 mM) AgNO3 concentrations respectively. Also the result of AFM height analysis of green synthetic nanoparticle indicated that the height were AgNO3 concentrations dependent in which (56.861, 13.636, 13.521 and 15.366 nm) for (1, 1.5, 1.75 and 2 nm) respectively. The antibacterial activity of green synthetic sliver nanoparticles was studied against one type of Gram negative (E. coli) and Gram positive (stapylococcus auerus) and the result showed that different nanoparticles concentrations (1, 1.5, 1.75, 2 mM) have the ability to inhibit the bacterial isolate with varying zones of (17, 20, 12,17mm) for Staphylococcus. auerus and (10,22,10,22 mm) for E. coli at (1, 1.5, 1.75, 2 mM) respectively.