INFLUENCES OF SILVER NANOPARTICLES In vitro MORPHOGENESIS OF SPECIALTY KING BANANA (Musa ssp.) IN VIETNAM

The King banana (Musa ssp.) is a native specialty variety in Vietnam with distinctive features, delicious taste specialty and high economic value. The objectives of this study were to investigate the effects of different concentrations of silver nanoparticles (AgNPs) on the callus formation, shoot regeneration and multiplication, root induction and nursery phase during in vitro propagation. The results have shown that approximately 98.0% of pseudostems were formed in the callus in the MS medium supplemented by 8.0 ppm of AgNPs after 3 weeks of culture. The calluses were then examined on the basal MS medium added with different AgNPs concentrations (0.0, 2.0, 4.0 and 6.0 ppm). The maximum of shoot numbers was found in the medium supplemented by 4.0 ppm after 4 weeks cultured. Moreover, the highest shoot regeneration rate was found in the stem culture supplemented by 6.0 ppm by 93.33% with a shooting coefficient of 4.22 value. Similarly, the medium supplemented with 4.0 ppm of AgNPs has been the best choice for rooting in vitro with a rate of 98.33%, an average of 5.22 roots per shoot, root length of 4.26 cm, respectively. The plantlets had remarkable characteristics in the nursery stage with a height of 8.17 cm, 1.68 leaves per plant. Our results have revealed that AgNPs have exerted strikingly positive influences on in vitro morphogenesis of the King banana. We have also discussed the complex effects of AgNPs induced on plant growth in vitro which show not only AgNPs concentrations-culture medium dependent but also part of organ and plant species uses dependent, respectively. Our findings may open a new window for using AgNPs to conserve both the specialty fruits and endanger species in vitro in the future.