Study of Different In-vitro Methods for Thrombolytic Activity

During the study and development of novel thrombolytic agent, it is an important consideration to evaluate the Safety and efficacy of thrombolytic drugs, which are standard treatment option for myocardial infarction and ischemic stroke. Different methods for measuring thrombolytic activity are Spectrophotometric analysis of thrombolytic activity (SATA) assay, Micro centrifuged method, Clot lysis, Pro thrombin time (PTT), global thrombosis test but they has some drawbacks, including being expensive, time-consuming, difficult and inaccurate. We reviewed the research papers related to thrombolytic activity studies by searching the internet using Google Scholar and the Pub Med database. Utilizing the pertinent search terms (thrombolytic activity + plant extract + In-vitro methods) in the month of January 2023. 24 research articles out of 36 were subjected to an initial review. Our current work identified the frequency of use of the five in vitro techniques, the order of utilization was clot lysis method, Prothrombin time, micro centrifuged method, Spectrophotometric analysis, and Global thrombosis test. Hence Clot lysis method was considered as the easiest, simplest, and least expensive in vitro technique; as a result, it may have been used the majority of the time to assess a sample's thrombolytic activity.