Agrobacterium-mediated Transformation and Plant Regeneration from Different Explants of Lycopersicon esculentum

Kalbande, B.B. and Vyas, A. and Yadav, A.M. and Narwade, A.V. and Nandeshwar, S.B. (2012) Agrobacterium-mediated Transformation and Plant Regeneration from Different Explants of Lycopersicon esculentum. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 13 (1-2). pp. 53-58.

Full text not available from this repository.

Abstract

Tomato is one of the most important and essential vegetable crops all over the world. The present study was focused on genetic transformation and plant regeneration of tomato (Lycopersicon esculentum cv PKM-1). Transformation was carried out by Agrobacterium tumifaciens harboring binary vector pBI121 containing modified Cry1Ac gene. Three explants namely cotyledonary node, cotyledonary leaves and hypocotyl was used in transformation. After co-cultivation, transformed explants were selected on Kanamycin medium (100 mg/l) and selected explants were cultured for regeneration into shoots. Regenerated shoots were rooted and plants were established. Some of the established plants were tested PCR positive with gene specific primers for the presence of Cry1Ac gene.

Item Type: Article
Subjects: Open Research Librarians > Biological Science
Depositing User: Unnamed user with email support@open.researchlibrarians.com
Date Deposited: 09 Jan 2024 05:22
Last Modified: 09 Jan 2024 05:22
URI: http://stm.e4journal.com/id/eprint/2260

Actions (login required)

View Item
View Item