Mutations in the FOXO3 Gene and Their Effects on Meat Traits in Gannan Yaks

Qi, Youpeng and Wang, Xiangyan and Zhu, Chune and Mi, Baohong and Cui, Changze and Chen, Shaopeng and Zhao, Zhidong and Zhao, Fangfang and Liu, Xiu and Wang, Jiqing and Shi, Bingang and Hu, Jiang (2024) Mutations in the FOXO3 Gene and Their Effects on Meat Traits in Gannan Yaks. International Journal of Molecular Sciences, 25 (4). p. 1948. ISSN 1422-0067

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Abstract

The FOXO3 gene, a prominent member of the FOXO family, has been identified as a potential quantitative trait locus for muscle atrophy and lipid metabolism in livestock. It is also considered a promising candidate gene for meat quality traits such as Warner–Bratzler shear force (WBSF) and water holding capacity (WHC). The aim of this study was to identify sequence mutations in the FOXO3 gene of yaks and to analyze the association of genotypes and haplotypes with meat traits such as WBSF and WHC. Quantitative reverse-transcriptase PCR (RT-qPCR) was applied to determine the expression levels of FOXO3 in yak tissues, with the results revealing a high expression in the yak longissimus dorsi muscle. Exons of the FOXO3 gene were then sequenced in 572 yaks using hybrid pool sequencing. Five single nucleotide polymorphisms were identified. Additionally, four effective haplotypes and four combined haplotypes were constructed. Two mutations of the FOXO3 gene, namely C>G at exon g.636 and A>G at exon g.1296, were associated with cooked meat percentage (CMP) (p < 0.05) and WBSF (p < 0.05), respectively. Furthermore, the WBSF of the H2H3 haplotype combination was significantly lower than that of other combinations (p < 0.05). The findings of this study suggest that genetic variations in FOXO3 could be a promising biomarker for improving yak meat traits.

Item Type: Article
Subjects: Open Research Librarians > Multidisciplinary
Depositing User: Unnamed user with email support@open.researchlibrarians.com
Date Deposited: 06 Feb 2024 11:44
Last Modified: 06 Feb 2024 11:44
URI: http://stm.e4journal.com/id/eprint/2470

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