Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Chen, Kang and Hao, Jia and Noritake, Kanako and Yamashita, Yu and Kuroda, Shinji and Kasugai, Shohei (2013) Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model. Open Journal of Regenerative Medicine, 02 (01). pp. 8-14. ISSN 2169-2513

[thumbnail of OJRM_2013032913581783.pdf] Text
OJRM_2013032913581783.pdf - Published Version

Download (748kB)

Abstract

Purpose: Low intensity pulsed ultrasound stimulation (LIPUS) has been clinically applied to promote bone fracture healing in the orthopedic field. Thus, it is likely that LIPUS also stimulates bone regeneration in bone defects in the cranial-maxillofacial area. However, this has not been clearly proved. Furthermore, optimal time point and period of the application after the surgery has not been reported. The purpose of the present study was to evaluate the effect of LIPUS on bone regeneration in the rat parietal bone defects especially focusing on time and period of the application. Materials and Methods: Eighteen Wistar rats (14 weeks old) were divided into 6 groups: 5 experimental groups and a control group. Bone defect of 5 mm diameter was prepared on each side of the parietal bone and customized gelatin membranes were placed over the bone defects. LIPUS (160 mW/cm2, 15 min/day) was applied to the defect area with an active transducer externally in the experimental groups according to the schedules of the applications: Group 1 (day 6 - 12), group 2 (day 13 - 19), group 3 (day 20 - 26), group 4 (day 6 - 19) and group 5 (day 6 - 26). All the animals were sacrificed at 28 days. The defects were analyzed with micro CT and then histologically. Results: In Group 1, new bone formation was significantly promoted and the newly-formed bone was thick and matured compared to the one of the control group. In other experimental groups there were tendencies of stimulation of new bone formation; however, they were not statistically significant. Discussion and Conclusion: The present study demonstrated that amount of new bone formation in the bone defect depended on the time and period of LIPUS application. It has been suggested that application of LIPUS at an early healing period, the second week after the surgery, effectively accelerated new bone formation.

Item Type: Article
Subjects: Open Research Librarians > Biological Science
Depositing User: Unnamed user with email support@open.researchlibrarians.com
Date Deposited: 14 Mar 2023 12:29
Last Modified: 11 May 2024 08:59
URI: http://stm.e4journal.com/id/eprint/351

Actions (login required)

View Item
View Item