Sodium Butyrate as Adjuvant Therapy with Mycophenolate Mofetil for Amelioration of Skin Fibrosis in Bleomycin-induced Scleroderma in Mice via Immunomodulatory, Antioxidant, Antiapoptotic and Antifibrotic Mechanisms

Othman, Qamar and Abdelmaaboud, Maaly A. and Abdin, Amany A. and Fathi, Fleur (2022) Sodium Butyrate as Adjuvant Therapy with Mycophenolate Mofetil for Amelioration of Skin Fibrosis in Bleomycin-induced Scleroderma in Mice via Immunomodulatory, Antioxidant, Antiapoptotic and Antifibrotic Mechanisms. Journal of Advances in Medicine and Medical Research, 34 (21). pp. 259-271. ISSN 2456-8899

[thumbnail of sciencedomain,+Othman34212022JAMMR90766.pdf] Text
sciencedomain,+Othman34212022JAMMR90766.pdf - Published Version

Download (1MB)

Abstract

Background: Scleroderma is an autoimmune, multi-organ disease; its clinical hallmark is cutaneous and systemic fibrosis. Up to date, there is no definite cure.

Aim of the Study: The aim of this study was to investigate the potential effects of sodium butyrate (NaB), and mycophenolate mofetil (MMF) each alone or both in a mice model of scleroderma induced by bleomycin (BLM), and to clarify some of their possible mechanisms on oxidative stress, immunological pattern, apoptosis, inflammation and fibrosis.

Methods: This study was carried out on 50 Balb/C mice, divided into 5 equal groups. Serum levels of anticentromere antibody (ACA), and skin tissue levels of the following parameters were assayed: transforming growth factor-β1 (TGF-β1), interleukin-4 (IL-4), malondialdehyde (MDA), and superoxide dismutase (SOD). Tissue sections were examined for histopathological changes, dermal thickness, and immunohistochemical expression of caspase- 3, and α-smooth muscle actin (α-SMA).

Results and Conclusion: The combined group showed a significant decline in TGF-β1, IL-4, MDA levels, caspase-3, and α-SMA, as well as a significant increase in SOD. Moreover, marked reduction in dermal fibrosis and dermal thickness compared to monotherapy by either NaB or MMF. The combination of NaB and MMF is a promising candidate for the treatment of scleroderma.

Item Type: Article
Subjects: Open Research Librarians > Medical Science
Depositing User: Unnamed user with email support@open.researchlibrarians.com
Date Deposited: 13 Mar 2023 12:14
Last Modified: 12 Jan 2024 07:27
URI: http://stm.e4journal.com/id/eprint/365

Actions (login required)

View Item
View Item