NS-398 induces caspase-dependent, mitochondria-mediated intrinsic apoptosis of hepatoma cells

Song, Il Han and Kim, Suk Bae and Shin, Hyun Duk and Kang, Ha Yan and Kim, Eun Young (2012) NS-398 induces caspase-dependent, mitochondria-mediated intrinsic apoptosis of hepatoma cells. Advances in Bioscience and Biotechnology, 03 (06). pp. 649-656. ISSN 2156-8456

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Abstract

The present study was conducted to investigate whether mitochondrial pathway of apoptosis is involved in cyclooxygenase-2 (COX-2) inhibitor-induced growth inhibition of hepatoma cells. The growth rate and pattern of NS-398 (selective COX-2 inhibitor)-treated Hep3B hepatoma cells were analyzed by microscopic examination, DNA fragmentation gel analysis and flow cytometry followed by the cleavage of down-stream caspase 3 and the release of cytosolic fraction of cytochrome c assessed by Western blot analysis. NS-398 induced the growth inhibition of hepatoma cells depending on the concentration of this COX-2 inhibitor and time sequence. Ladder patterned-DNA fragmentation and cytometric redistribution to sub-G1 phase in cell cycle were revealed in NS-398-induced growth inhibition of hepatoma cells. Cytochrome c was translocated from mitochondria to cytosol in time-dependent manner following NS-398 treatment to hepatoma cells. COX-2 inhibitor induces the growth inhibition of hepatoma cells via caspase-dependent, mitochondria-mediated intrinsic apoptosis pathway. These results strongly suggest the possibility of therapeutic implication of COX-2 inhibitor in HCC.

Item Type: Article
Subjects: Open Research Librarians > Biological Science
Depositing User: Unnamed user with email support@open.researchlibrarians.com
Date Deposited: 22 Mar 2023 10:07
Last Modified: 04 May 2024 04:45
URI: http://stm.e4journal.com/id/eprint/440

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