Álvarez, Jimena Inés Ruiz and Teijeiro, Juan Manuel and Marini, Patricia Estela (2011) Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity. Advances in Bioscience and Biotechnology, 02 (02). pp. 85-88. ISSN 2156-8456
ABB20110200011_93311269.pdf - Published Version
Download (796kB)
Abstract
Immunological methods involving pregnancy associ-ated glycoproteins (PAG) are used for cattle preg-nancy detection. The faults of these methods could be overcomed by using antibodies specific for each member of the PAG family. In order to differentiate between very similar proteins, preparation of anti-bodies specific for peptides is a method of choice. In this work, we summarize a series of considerations regarding peptide design and choose free access NCBI, Antigenicity Plot, EMBOSS Antigenic and Expasy tools to apply them. We design peptides spe-cific for different reported PAG members and obtain the corresponding polyclonal antibodies for five of them.
Item Type: | Article |
---|---|
Subjects: | Open Research Librarians > Biological Science |
Depositing User: | Unnamed user with email support@open.researchlibrarians.com |
Date Deposited: | 23 Mar 2023 09:36 |
Last Modified: | 23 Apr 2024 12:12 |
URI: | http://stm.e4journal.com/id/eprint/459 |