Chemiluminescence based immunoassay for the detection of heroin and its metabolites

Singh, Smritee and Mishra, Priya and Banga, Ivneet and Parmar, Avanish S and Tripathi, Prem Prakash and Gandhi, Sonu (2017) Chemiluminescence based immunoassay for the detection of heroin and its metabolites. BioImpacts, 8 (1). pp. 53-58. ISSN 2228-5660

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Abstract

Introduction: Continuous use of opiates causes drug-related illnesses, which poses an alarming situation to develop sensitive detection platform. In this study, a highly sensitive and reliable chemiluminescence immunoassay (CI) has been developed for the detection of heroin and its major metabolites in spiked urine samples.

Methods: To develop robust immunoassay, monoacetyl morphine-bovine serum albumin (MAM-BSA) conjugate was synthesized and characterized thoroughly by physicochemical techniques. The anti-MAM antibodies were developed, labeled with horseradish peroxidase (HRP) and immunoassay was developed to detect the presence of target drug in spiked urine samples.

Results: A competitive CI was developed, where heroin, MAM, morphine, and codeine concentration were ranged from 0-1000 ng/ mL in spiked urine samples and limit of detection were 80, 95, 90, 75 pg/ mL.

Conclusion: The developed CI is highly sensitive, specific, point of care, cost-effective and can be used as a routine technique for quantitative analysis for screening of narcotic drugs.

Item Type: Article
Subjects: Open Research Librarians > Medical Science
Depositing User: Unnamed user with email support@open.researchlibrarians.com
Date Deposited: 31 Mar 2023 08:08
Last Modified: 06 Nov 2023 05:02
URI: http://stm.e4journal.com/id/eprint/526

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