Bio-waste Transesterification Alternative for Biodiesel Production: A Combined Manipulation of Lipase Enzyme Action and Lignocellulosic Fermented Ethanol

Udeh, Benard (2018) Bio-waste Transesterification Alternative for Biodiesel Production: A Combined Manipulation of Lipase Enzyme Action and Lignocellulosic Fermented Ethanol. Asian Journal of Biotechnology and Bioresource Technology, 3 (3). pp. 1-9. ISSN 24570125

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Abstract

Petroleum hydrocarbon oil contaminated soils were excavated from a gas station from Nicosia Cyprus, Turkey and subjected to fungal isolation/analysis. During the fungal essay, Aspergillus nigar, Penicellum notatum and Mucorales were isolated and identified. Aspergillus nigar was selected and cultivated for lipase enzyme production due to its role in lipase production. The produced lipase enzyme was immobilized and the number of lipases recovered after purification was 85.1%. The purified enzymes that were supported by physical adsorption which resulted in high (85%) yield showed high stability than free lipase, encouraged transesterification in this study. Pretreatment evaluation of groundnut powder showed high cellulose yield (82.3% and 87%) and high lignin removal of 75%, 88% respectively for lipase enzyme accessibility and ethanol production. Transesterification involving purified lipases and groundnut shell fermented ethanol yielded Methyl ester which represents the organic biodiesel with a C18 number showing a minimum level of lubricity for friction reduction between surfaces when a car is on motion and has specific gravity 0.95 ul, density 15.5°c, and calorific value of 43.2 MJ. Kg.

Item Type: Article
Subjects: Open Research Librarians > Biological Science
Depositing User: Unnamed user with email support@open.researchlibrarians.com
Date Deposited: 09 May 2023 09:56
Last Modified: 14 Mar 2024 04:47
URI: http://stm.e4journal.com/id/eprint/779

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