Assessment of different samples for molecular diagnosis of extra-pulmonary tuberculosis

Gasmelseed, N. and Aljak, M. A. and Elmadani, A. E. and Elgaili, M. E. and Saeed, O. K. (2014) Assessment of different samples for molecular diagnosis of extra-pulmonary tuberculosis. International Journal of Biotechnology and Molecular Biology Research, 5 (5). pp. 41-47. ISSN 2141-2154

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Abstract

Extrapulmonary tuberculosis is an important clinical problem particularly in developing countries. The aim of this study was to assess two different samples (blood and fluid) for the diagnosis of extra-pulmonary tuberculosis (abdominal tuberculosis and tuberculous lymphadenitis). The study subjects were recruited from WadMedani Teaching Hospital during 2009-2013. Seventy five ascetic fluid and blood samples were collected from each suspected patient with abdominal tuberculosis and twenty five lymphatic aspirates and blood samples were collected from each suspected tuberculous lymphadenitis patient. DNA was extracted using DNPTM kit (CinnaGenInc) and polymerase chain reaction (PCR) was done using IS6110 gene for both samples. In abdominal tuberculosis, 20/75 (27%) were positive for tuberculosis when ascetic fluid was used and 9/75 (12%) in case of blood samples. The comparison between ascetic fluid and blood samples, showed that, there was a significant difference in both results, P-value < 0.05. In tuberculous lymphadenitis, 13/25 (52%) and 3/25 (12%) were positive to tuberculosis when lymph aspirate and blood were used respectively. This study concluded that the best sample for diagnosis of abdominal TB and lymphadenitis is ascetic fluid and LN aspirate. This study recommends that ascetic fluid and lymph aspirate samples are recommended to be used in molecular diagnostic test.

Item Type: Article
Subjects: Open Research Librarians > Agricultural and Food Science
Depositing User: Unnamed user with email support@open.researchlibrarians.com
Date Deposited: 10 May 2023 10:05
Last Modified: 03 Feb 2024 04:38
URI: http://stm.e4journal.com/id/eprint/908

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