In vitro Antioxidant Activity and Total Polyphenols Content of Wild Edible Polypore Mushrooms – Bondazewia berkeleyi and Ganoderma lucidum

Aim: To determine the total phenols content and antioxidant activity of B. berkeleyi and G. lucidummethanol extracts.
Study Design: In vitro evaluation of antioxidant assays and quantitative determination of total phenolics and flavonoids content of B. berkeleyi and G. lucidum mushroom extracts.
Place and Duration of Study: Department of Chemistry, University of Uyo, Nigeria (August – November, 2014).
Methodology: The total phenolics content was estimated using Folin-Ciocalteu method and total flavonoid content was determined using aluminum chloride. In vitro antioxidant activities of extracts were studied in different systems including 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay and metal chelating activity along with standards.
Results: Total phenols content in B. berkeleyi and G. lucidum was 32.99 and 42.7 mg GAE/g and flavonoids amounted to 22.4 and 35.23 mg QE/g respectively. The extracts of B. berkeleyi and G. lucidum exhibited significant DPPH radical scavenging activity with IC50 values 22.9 μg/ml and 14.6 μg/ml; metal chelating capacity of 67.4% and 82.6% at 500 μg/mL respectively.
Conclusion: The results of this study substantiates the use of the extract of G. lucidum powder as an antioxidant agent and serves as a pointer for further exploitation of B. berkeleyi mushroom for its antioxidant compounds and other potential biological activity.