Karrat, Lina and Nayal, Ream and Abajy, Mohammad Yaser (2020) Chemical Composition of Pinus brutia Ten Essential Oil and Its in vitro Anti-Inflammatory Activity. International Research Journal of Pure and Applied Chemistry, 21 (24). pp. 112-121. ISSN 2231-3443
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Abstract
This investigation aims to determine the chemical composition of Pinus brutia leaves essential oil and evaluate its anti-inflammatory property using Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay. The chemical composition of essential oil (EO) obtained by hydro-distillation of leaves of Pinus brutia was investigated by GC-MS. The anti-inflammatory effect of EO was evaluated using Human Red Blood Cells (HRBC) membrane stabilization assay and Albumin denaturation assay. The main constituents of EO were α-Terpineol (66.16%), 3-Carene (4.90%), Carveol (4.55%) and cis-Verbenol (3.22%). The inhibion of hemolysis was observed at concentrations (2.5-12.5) µg/ml. Moreover, albumin denaturation test showed protection effect at concentrations (8-40) µg/ml. We concluded that, Pinusbrutia EO shows strong anti-inflammatory activity at different concentration when compared to standard drug of Diclofenac sodium. In addition, GC-MS analysis of Pinus brutia EO showed the presence of α-Terpineol as major compound in the oil. It reveals that this constituent is responsible to maximum protection of albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory by in vivo models.
Item Type: | Article |
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Subjects: | Open Research Librarians > Chemical Science |
Depositing User: | Unnamed user with email support@open.researchlibrarians.com |
Date Deposited: | 20 Feb 2023 10:06 |
Last Modified: | 16 Feb 2024 04:21 |
URI: | http://stm.e4journal.com/id/eprint/188 |